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Biocare Medical intellipath flxtm autostainer
Intellipath Flxtm Autostainer, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intellipath+flxtm+autostainer/autostainer+flx+intellipath/pmc12706006-271-8-11
Average 86 stars, based on 1 article reviews
intellipath flxtm autostainer - by Bioz Stars, 2026-11
86/100 stars

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Staining:

Article Title: Circulating tumor cell and oncosome subtypes in portal and peripheral venous circulations may be used for diagnosis and prognostication of pancreatic cancer.
Article Snippet: .. Blood Sample Staining and Imaging Two slides per sample test were in the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) as previously described [31, 43-46]. .. Samples were fixed with 2% paraformaldehyde and stained with anti-human CD31: Alexa Fluor 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, California, USA; RRID:AB_322463), anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, Philadelphia, USA), and permeabilized with 100% cold methanol.

Article Title: Determining the efficacy of ExThera Seraph100 blood filtration in patients diagnosed with pancreatic cancer through the liquid biopsy
Article Snippet: .. At room temperature, slides were processed in the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) and were stained with 2.5 μg/mL of a mouse IgG1 anti-human CD31: Alexa Fluor 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, California, USA; RRID:AB_322463), 100 μg/mL of a goat anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, Philadelphia, USA), and were permeabilized with 100% cold methanol. .. This was followed by an antibody cocktail including: mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA, USA), rabbit IgG anti-human vimentin (Vim) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA, USA; RRID:AB_10829352), mouse anti-human CD45:Alexa Fluor® 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA; RRID:AB_324730), and IgG1/Ig2a anti-human cytokeratins (CKs) 1, 4, 5, 6, 8, 10, 13, 18, and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2, C2562, Sigma, St. Louis, MO, USA; RRID:AB_476839).

Article Title: Cancer-related cells and oncosomes in the liquid biopsy of pancreatic cancer patients undergoing surgery
Article Snippet: .. The slides were stained at room temperature using the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) according to the Landscape immunofluorescence (IF) protocol as described previouslye – , . .. Briefly, after fixation with 2% paraformaldehyde, slides are incubated with 2.5 ug/mL of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA) and 100 ug/mL of a goat anti-mouse IgG monoclonal Fab fragments (115-007- 003, Jackson ImmunoResearch, West Grove, PA, USA).

Article Title: Characterizing circulating rare cells in peripheral blood for detecting and monitoring multiple myeloma and precursor states.
Article Snippet: .. Two slides per sample were thawed prior to immunofluorescent (IF) staining using an IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) as previously described35. ..

Article Title: Characterizing circulating rare cells in peripheral blood for detecting and monitoring multiple myeloma and precursor states
Article Snippet: .. Two slides per sample were thawed prior to immunofluorescent (IF) staining using an IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) as previously described . ..

Imaging:

Article Title: Circulating tumor cell and oncosome subtypes in portal and peripheral venous circulations may be used for diagnosis and prognostication of pancreatic cancer.
Article Snippet: .. Blood Sample Staining and Imaging Two slides per sample test were in the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) as previously described [31, 43-46]. .. Samples were fixed with 2% paraformaldehyde and stained with anti-human CD31: Alexa Fluor 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, California, USA; RRID:AB_322463), anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, Philadelphia, USA), and permeabilized with 100% cold methanol.

Immunofluorescence:

Article Title: Cancer-related cells and oncosomes in the liquid biopsy of pancreatic cancer patients undergoing surgery
Article Snippet: .. The slides were stained at room temperature using the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) according to the Landscape immunofluorescence (IF) protocol as described previouslye – , . .. Briefly, after fixation with 2% paraformaldehyde, slides are incubated with 2.5 ug/mL of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA) and 100 ug/mL of a goat anti-mouse IgG monoclonal Fab fragments (115-007- 003, Jackson ImmunoResearch, West Grove, PA, USA).



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Biocare Medical intellipath flxtm autostainer
Intellipath Flxtm Autostainer, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intellipath+flxtm+autostainer/autostainer+flx+intellipath/pmc12706006-271-8-11
Average 86 stars, based on 1 article reviews
intellipath flxtm autostainer - by Bioz Stars, 2026-11
86/100 stars
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